Monoclonal Anti-β-Tubulin antibody produced in mouse T5293 from MilliporeSigma

Product Specs
ItemMonoclonal Anti-β-Tubulin antibody produced in mouse
CompanyMilliporeSigma
Price Supplier Page View Company Product Page
Catalog NumberT5293
NCBI Full Gene Nametubulin beta 2A class IIa, tubulin beta 1 class VI, tubulin, beta 4B class IVb, tubulin beta 4B class IVb
NCBI Gene AliasesCDCBM5, Tubb2, TUBB2, TUBB, LCAEOD, TUBB2C, Beta2, Tubb2c
Applicationswestern blot, indirect immunofluorescence
ReactivityHu, Rt, Bv, C.el, Ck, Inv, SeUr
Clonalitymonoclonal
Antibody Typeprimary antibodies
Hostmouse
Clone2-28-33
TargetTUBB1; rat Tubb2c
Conjugate/Tagunconjugated

Add to Compare List

MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) Critical requirement of SOS1 for tumor development and microenvironment modulation in KRASG12D-driven lung adenocarcinomaNature CommunicationsSeptember 20, 2023Fernando C. Baltanás, Rósula García-Navas, Pablo Rodríguez-Ramos, Nuria Calzada, Cristina Cuesta, Javier Borrajo, Rocío Fuentes-Mateos, Andrea Olarte-San Juan, Nerea Vidaña, Esther Castellano, et al.(Cat#610096, 1:500, BD), anti-SOS2 (Cat#sc-15358, 1:500, Santa Cruz Biotechnology), anti-tubulin (Cat#T5293, 1:10,000, Sigma) and anti-vinculin (Cat#26520-1-AP, 1:5000, ProteinTech) and the corresponding secondary

(2) Mechanisms of synergistic suppression of ALK-positive lung cancer cell growth by the combination of ALK and SHP2 inhibitorsScientific ReportsJune 20, 2023M. A. Berry, A. R. Bland, J. C. AshtonPA5-64,482) were purchased from Invitrogen (MA, USA). The ERK1/2 (9102S), CDK1 (HPA003387), and β-tubulin (T5293) antibodies were purchased from Sigma-Aldrich (St Louis, MO, USA). The secondary antibody horseradish

(3) The Antibiotic Kitasamycin—A Potential Agent for Specific Fibrosis Preventing Therapy after Fistulating Glaucoma Surgery?PharmaceuticsJanuary 18, 2023Katharina A. Sterenczak, Georg Fuellen, Anselm Jünemann, Rudolf F. Guthoff, Oliver Stachs, Thomas Stahnkeanti-α-SMA (ab7817 (1: 500); Abcam), anti-β-actin (A2228 (1:500); Sigma-Aldrich) and anti-β-tubulin (T5293 (1:250); Sigma-Aldrich). Subsequently, membranes were washed (3×) with TBS spiked with Tween 20 (0.1%)

(4) Photon-free (s)CMOS camera characterization for artifact reduction in high- and super-resolution microscopyNature CommunicationsDecember 1, 2022Robin Diekmann, Joran Deschamps, Yiming Li, Takahiro Deguchi, Aline Tschanz, Maurice Kahnwald, Ulf Matti, Jonas Riessamples had been washed three times with PBS, microtubules were labeled with anti-β-tubulin antibody (T5293; Sigma-Aldrich), diluted 1:300 in PBS with 2% (w/v) BSA, for 2 h. After being washed three times with

(5) PU.1-c-Jun interaction is crucial for PU.1 function in myeloid developmentCommunications BiologySeptember 14, 2022Xinhui Zhao, Boris Bartholdy, Yukiya Yamamoto, Erica K. Evans, Meritxell Alberich-JordÃ , Philipp B. Staber, Touati Benoukraf, Pu Zhang, Junyan Zhang, Bon Q. Trinh, et al.with anti-PU.1 antibody (Santa Cruz sc-352) to measure PU.1 protein expression. Anti-β-tubulin (Sigma T5293 clone 2-28-33) was used to ensure the even loading of protein extracts. Retroviral transduction of

(6) Biochemical analysis of novel NAA10 variants suggests distinct pathogenic mechanisms involving impaired protein N-terminal acetylationHuman geneticsAugust 1, 2022McTiernan N, Tranebjærg L, Bjørheim AS, Hogue JS, Wilson WG, Schmidt B, Boerrigter MM, Nybo ML, Smeland MF, Tümer Z, et al.dilution, Invitrogen R960-25), β-tubulin mouse monoclonal antibody (1:3000 dilution, Sigma-Aldrich T5293) and NAA15 rabbit polyclonal antibody (1:2000, Biogenes, previously called anti-NATH) (Arnesen et al.

(7) Role of Nuclear Lamin A/C in the Regulation of Nav1.5 Channel and Microtubules: Lesson From the Pathogenic Lamin A/C Variant Q517XFrontiers in Cell and Developmental BiologyJune 29, 2022Roberta De Zio et al.2016). Primary antibodies used were: anti-β-Tubulin antibody produced in mouse (1:1,000 in PBS‐BSA 1%, T5293 Sigma-Aldrich), the anti-mCherry antibody (1:500 in PBS‐BSA 1%, ab167453 Abcam), the anti-γ-tubulin

(8) Decreased anterograde transport coupled with sustained retrograde transport contributes to reduced axonal mitochondrial density in tauopathy neuronsFrontiers in molecular neuroscienceJanuary 1, 2022Sabui A, Biswas M, Somvanshi PR, Kandagiri P, Gorla M, Mohammed F, Tammineni P.(#ab9097, Abcam, United Kingdom), KIF1C (#20790-1-AP, Proteintech, Planegg-Martinsried, Germany), Tubulin (#T5293, Sigma, St. Louis, MO, United States). Cell culture and DNA transfection COS7 cells were used to over-express

(9) Direct supercritical angle localization microscopy for nanometer 3D superresolutionNature CommunicationsDecember 1, 2021Anindita Dasgupta, Joran Deschamps, Ulf Matti, Uwe Hübner, Jan Becker, Sebastian Strauss, Ralf Jungmann, Rainer Heintzmann, Jonas Riescoverslips upside down onto a drop of primary antibody staining mix (mouse-anti-beta-tubulin, catalog no. T5293, Sigma–Aldrich, diluted 1:300 in PBS containing 2% (w/v) BSA) for 2–3 h at RT. Weakly and unbound primary

(10) Deep learning enables fast and dense single-molecule localization with high accuracyNature MethodsSeptember 1, 2021Artur Speiser, Lucas-Raphael Müller, Philipp Hoess, Ulf Matti, Christopher J. Obara, Wesley R. Legant, Anna Kreshuk, Jakob H. Macke, Jonas Ries, Srinivas C. TuragaNeoMarkers, Fremont, CA, USA), and for ultra-high labeling (Fig. 4g) additionally with anti-β-tubulin (T5293; Sigma-Aldrich), each diluted 1:50 in PBS with 2% (w/v) BSA, overnight. After being washed three times

(11) Functional Specificity of the Members of the Sos Family of Ras-GEF Activators: Novel Role of Sos2 in Control of Epidermal Stem Cell HomeostasisCancersApril 29, 2021Baltanás FC, Mucientes-Valdivieso C, Lorenzo-Martín LF, Fernández-Parejo N, García-Navas R, Segrelles C, Calzada N, Fuentes-Mateos R, Paramio JM, Bustelo XR, et al.rabbit anti-Sos2 (sc-15358, 1:500, Santa Cruz Biotechnology, Dallas, TX, USA), and mouse anti-tubulin (T5293, 1:10,000, Sigma, St. Louis, MO, USA). 2.4. Microarray Analysis RNA from GFP-Adeno-Cre-treated 2D-cultured

(12) Elevated CXorf67 Expression in PFA Ependymomas Suppresses DNA Repair and Sensitizes to PARP InhibitorsCancer cellDecember 14, 2020Han J, Yu M, Bai Y, Yu J, Jin F, Li C, Zeng R, Peng J, Li A, Song X, et al.anti-RPA2,ABclonal,Cat# A2189; RRID: AB_2764207 Mouse monoclonal anti-β-Tubilin,Sigma-Aldrich,Cat# T5293; RRID: AB_477580 Bacterial and Virus Strains,Bacterial and Virus Strains,Bacterial and Virus Strains DH10Bac

(13) SLC25A51 is a mammalian mitochondrial NAD⁺ transporterNatureDecember 1, 2020Luongo TS, Eller JM, Lu MJ, Niere M, Raith F, Perry C, Bornstein MR, Oliphint P, Wang L, McReynolds MR, et al.anti-MTC02 (Abcam, #ab9479, 1:1000), anti-CoxIV (Abcam, #ab33985, 1:1000), anit-β-tubulin (Sigma, #T5293, 1:10,000) and anti-mouse IgG H&L IRDye® 800CW (Abcam, #ab216773, 1:10,000), IRDye® 680RD Goat anti-Mouse

(14) Efficient and crucial quality control of HAP1 cell ploidy statusBiology OpenNovember 15, 2020Tobias B. Beigl, Ine Kjosås, Emilie Seljeseth, Nina Glomnes, Henriette Aksneswere rabbit-anti-CoxIV (Cell Signalling Technologies, 4850) and mouse-anti-β-tubulin (Sigma-Aldrich, T5293). Coverslips were incubated for at least 1 hour, cell-side down, on drops of the antibody solution in

(15) Suppression of the TGF-β pathway by a macrolide antibiotic decreases fibrotic responses by ocular fibroblasts in vitroRoyal Society Open ScienceSeptember 16, 2020Thomas Stahnke et al.monoclonal anti-fibronectin, Sigma Aldrich, F7387 (1 : 500), mouse monoclonal anti-β-tubulin, Sigma Aldrich, T5293 (1 : 250), mouse monoclonal anti-β-actin, Sigma Aldrich, A2228 (1 : 500), mouse monoclonal anti-vimentin,

(16) Indoprofen prevents muscle wasting in aged mice through activation of PDK1/AKT pathwayJournal of Cachexia, Sarcopenia and MuscleAugust 1, 2020Hyebeen Kim, Sung Chun Cho, Hyeon‐Ju Jeong, Hye‐Young Lee, Myong‐Ho Jeong, Jung‐Hoon Pyun, Dongryeol Ryu, MinSeok Kim, Young‐Sam Lee, Minseok S. Kim, et al.(Cell Signaling Technology), Myoglobin (ab77232, 1:1000), T‐OXPHOS (ab110413, 1:1000) (Abcam), β‐tubulin (t5293, 1:1000; Sigma), Atrogin‐1 (sc‐33782, 1:1000), HSP90 (sc‐7947, 1:1000), p‐ATF2 (Thr71) (sc‐8398, 1:1000),

(17) Severe syndromic ID and skewed X-inactivation in a girl with NAA10 dysfunction and a novel heterozygous de novo NAA10 p.(His16Pro) variant - a case reportBMC medical geneticsJuly 22, 2020Bader I, McTiernan N, Darbakk C, Boltshauser E, Ree R, Ebner S, Mayr JA, Arnesen T.by Western blot using V5-tag antibody (1:5000, Invitrogen, #R960–25) and β-tubulin antibody (1:3000, Sigma, T5293). The amount of NAA10-V5 present at timepoints 2, 4 and 6 h post treatment was normalized to the amount

(18) The transcription factor E2A drives neural differentiation in pluripotent cellsDevelopment (Cambridge, England)June 22, 2020Rao C, Malaguti M, Mason JO, Lowell S.antibodies used in the western blot analysis were mouse anti-β-III-tubulin/Tuj1 (1:1000; Sigma-Aldrich, T5293), mouse anti-E2A (1:200; Santa Cruz Biotechnology, sc-416,), mouse anti-FLAG (1:5000; Sigma-Aldrich,

(19) ZNF746/PARIS overexpression induces cellular senescence through FoxO1/p21 axis activation in myoblastsCell Death & DiseaseMay 12, 2020Ju-Hyeon Bae, Hyeon-Ju Jeong, Hyebeen Kim, Young-Eun Leem, Dongryeol Ryu, Sang Chul Park, Yun-Il Lee, Sung Chun Cho, Jong-Sun Kang(Sigma-Aldrich, L8918), anti-p62 (Santa Cruz, sc-48402), and anti-β-tubulin antibody (Sigma-Aldrich, T5293). Then, samples were incubated with appropriate secondary antibodies conjugated with horseradish peroxidase

(20) GRAF2, WDR44, and MICAL1 mediate Rab8/10/11-dependent export of E-cadherin, MMP14, and CFTR ΔF508The Journal of cell biologyMay 4, 2020Lucken-Ardjomande Häsler S, Vallis Y, Pasche M, McMahon HT.(mouse, clone 8G5F11, Cat# EB0010, RRID:AB_2811223; Kerafast); β-tubulin (mouse, clone 2–28-33, Cat# T5293, RRID:AB_477580; Sigma); Actin (mouse, clone AC-15, Cat# ab6276, RRID:AB_2223210; Abcam); Integrin-β1

Be the First to Write a Review!

Monoclonal Anti-β-Tubulin antibody produced in mouse from MilliporeSigma

MilliporeSigma

Citations (115)

Reviews

Monoclonal Anti-β-Tubulin antibody produced in mouse from MilliporeSigma

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map